Molecular and Histopathology Core

Our Mission

To provide a broad array of state of the art technical and professional pathology related services critical to biomedical investigations conducted at Penn State Milton S. Hershey Medical Center.

We wish to provide the highest quality technical and professional interpretative services at the lowest cost to you in a timely fashion.  Any time you have questions, special needs, comments or complaints, please contact the director of the lab, Douglas B. Stairs, Ph.D., at dstairs@hmc.psu.edu or 717-531-6725.

Services Provided

Routine Histology – Processing, Embedding & Sectioning

  • Fixation and processing of human and animal tissues, as well as
    cultured cells and tissues Paraffin embedding and sectioning of
    tissues
  • Embedding and cryostat sectioning of fresh, frozen tissues
  • Questions:  Contact Kang Li at kxl30@psu.edu or 717-531-5876
     

Hematoxylin & Eosin StainingHematoxylin

  • Routine H&E staining of human and animal tissues,
    as well as cultured cells and tissues
  • H&E staining for paraffin or frozen sections
  • Questions:  Contact Kang Li at kxl30@psu.edu or 717-531-5876
     

 

 

Histochemical StainsHistochemical Stains

  • Histochemical staining of paraffin or frozen
  • tissue sections
  • PAS
  • Masson Trichrome
  • Toluidine Blue
  • Gomori's Aldehyde Fuchsin
  • Safranin O
  • Verhoeff Elastic
  • Cresyl Violet
  • Ability to develop and optimize histochemical stains
    for any tissue type or staining need
  • Questions:  Contact Kang Li at kxl30@psu.edu
    or 717-531-5876
     

Immunohistochemical StainsImmunoistochemical Stains

  • Immunohistochemical staining of human and animal
    tissues, as well as cultured cells and tissues
  • Ability to validate and develop protocols for any
    antibody
  • Mouse-on-mouse/rat immunohistochemistry
  • Chromogenic and fluorescent detection methods
  • Dual and triple IHC techniques
  • All IHC performed on an automated Ventana
    Discovery
    XT stainer
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044
     

In situ HybridizationIn situ Hybridization

  • Chromogenic OR fluorescent in situ hybridization
    protocols
  • Ability to develop and optimize protocols for your
    specific probes
  • All hybridizations protocols performed on an
    automated Ventana Discovery XT stainer
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044
     

 

 

Tissue MicroarrayTissue Microarray

  • Paraffin tissue microarrays (TMA's) can be created
  • with any combination of human tissue, animal tissues
    or cell culture pellets
  • Custom-sized and configured arrays are constructed
    using the Beecher MTA-I
  • Tissue core sizes can range from 0.6 mm to 2.0 mm,
    providing from 40 to over 200 sample cores in one
    paraffin block
  • Microarray slides can be stained using a variety of
    histochemical or immunohistochemical reactions
  • Questions:  Contact Trey Bruggeman at
    rbruggeman@hmc.psu.edu or 717-531-1044
     

PhotomicroscopyPhotomicroscopy

  • Create digital light microscopic images of your stained
    slides for presentation or publication.
  • Microscope system includes an Olympus BX51
    microscope outfitted with a DP25 digital camera
  • Ability to photography from 4x-60x
  • Photo editing software also available for minor
    image corrections
  • Questions:  Contact Trey Bruggeman
    at rbruggeman@hmc.psu.edu
    or 717-531-1044
     

Laser Capture Microdissection (LCM)Laser Capture Micodissection

  • Zeiss PALM Microbeam LCM
  • Capable of isolating cells, tissues, or stromal
    matrix from either frozen and paraffin sections
  • Ideal method to insure the purity of the starting
    material for downstream proteomic and genomic
    applications
  • Capable of operating on an RNAse free level to
    insure high quality samples for genomic studies
  • Questions:  Contact Trey Bruggeman at
    rbruggeman@hmc.psu.edu or 717-531-1044
     

Archival FFPE Human Tissue SamplesArchival FFPE Human Tissue Sample

  • Archival collection consisting of over 40 years of
    surgical pathology FFPE specimens
  • Searchable database enables the investigator to
    locate specific patient, tissue or tumor types
  • Ability to collaborate with anatomic pathologists to
    assist in the identification and selection of study
    samples along with the interpretation and scoring
    of results
  • Perfect for use as control tissues, study samples or
    TMA sources
  • Capable of isolating DNA or RNA from samples
  • Questions:  Contact Trey Bruggeman at
    rbruggeman@hmc.psu.edu or 717-531-1044
     

Douglas B. Stairs, Ph.D., Director