Molecular and Histopathology Core

Our Mission

To provide a broad array of state of the art technical and professional pathology related services critical to biomedical investigations conducted at Penn State Milton S. Hershey Medical Center.

We wish to provide the highest quality technical and professional interpretative services at the lowest cost to you in a timely fashion.  Any time you have questions, special needs, comments or complaints, please contact the director of the lab, Douglas B. Stairs, Ph.D., at dstairs@hmc.psu.edu or 717-531-6725.

Services Provided

Hematoxylin

Routine Histology – Processing, Embedding, Sectioning, and Hematoxylin and Eosin Staining

  • Fixation and processing of human and animal tissues, as well as cultured cells and tissues Paraffin embedding and sectioning of tissues
  • Embedding and cryostat sectioning of fresh, frozen tissues
  • Routine H&E staining of human and animal tissues, as well as cultured cells and tissues
  • H&E staining for paraffin or frozen sections
  • Questions:  Contact Kang Li at kxl30@psu.edu or 717-531-5876
   

Histochemical Stains

Histochemical Stains

  • Histochemical staining of paraffin or frozen tissue sections
  • PAS
  • Masson Trichrome
  • Toluidine Blue
  • Gomori's Aldehyde Fuchsin
  • Safranin O
  • Verhoeff Elastic
  • Cresyl Violet
  • Ability to develop and optimize histochemical stains for any tissue type or staining need
  • Questions:  Contact Kang Li at kxl30@psu.edu
    or 717-531-5876

Immunoistochemical Stains

Immunohistochemical Stains

  • Immunohistochemical staining of human and animal tissues, as well as cultured cells and tissues
  • Ability to validate and develop protocols for any antibody
  • Mouse-on-mouse/rat immunohistochemistry
  • Chromogenic and fluorescent detection methods
  • Dual and triple IHC techniques
  • All IHC performed on an automated Ventana Discovery XT stainer
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

In situ Hybridization

In situ Hybridization

  • Chromogenic OR fluorescent in situ hybridization protocols
  • Ability to develop and optimize protocols for your specific probes
  • All hybridizations protocols performed on an automated Ventana Discovery XT stainer
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

Tissue Microarray

Tissue Microarray

  • Paraffin tissue microarrays (TMA's) can be created with any combination of human tissue, animal tissues
    or cell culture pellets
  • Custom-sized and configured arrays are constructed using the Beecher MTA-I
  • Tissue core sizes can range from 0.6 mm to 2.0 mm, providing from 40 to over 200 sample cores in one
    paraffin block
  • Microarray slides can be stained using a variety of histochemical or immunohistochemical reactions
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

Photomicroscopy

Photomicroscopy

  • Create digital light microscopic images of your stained slides for presentation or publication.
  • Microscope system includes an Olympus BX51 microscope outfitted with a DP25 digital camera
  • Ability to photography from 4x-60x
  • Photo editing software also available for minor image corrections
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

Laser Capture Micodissection

Laser Capture Microdissection (LCM)

  • Zeiss PALM Microbeam LCM
  • Capable of isolating cells, tissues, or stromal matrix from either frozen and paraffin sections
  • Ideal method to insure the purity of the starting material for downstream proteomic and genomic
    applications
  • Capable of operating on an RNAse free level to insure high quality samples for genomic studies
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

Archival FFPE Human Tissue Sample

Archival FFPE Human Tissue Samples

  • Archival collection consisting of over 40 years of surgical pathology FFPE specimens
  • Searchable database enables the investigator to locate specific patient, tissue or tumor types
  • Ability to collaborate with anatomic pathologists to assist in the identification and selection of study samples along with the interpretation and scoring of results
  • Perfect for use as control tissues, study samples or TMA sources
  • Capable of isolating DNA or RNA from samples
  • Questions:  Contact Trey Bruggeman at rbruggeman@hmc.psu.edu or 717-531-1044

 

Douglas B. Stairs, Ph.D., Director