Resources & Technical

Histology and Histochemistry

  • Fixation and processing of human or animal tissues, cell culture flasks or pellets
  • Paraffin embedding and sectioning of tissues
  • Hematoxylin and eosin (H&E) staining of sections
  • Embedding, cryostat sectioning and staining of fresh, frozen tissues
  • Customized histochemical stains

 

Immunohistochemistry

  • Immunohistochemical staining of human or animal tissues, cell culture flasks or cell culture pellets
  • Mouse-on-mouse/rat immunohistochemistry
  • Chromogenic and fluorescent detection methods
  • Multiple antigen labeling techniques
  • Automated immunohistochemical staining performed on a DakoCytomation Autostainer Plus, for large volume studies
  • Professional assistance in the identification of the optimal commercial or research sources for antibodies and identification of best control tissues

 

In situ Hybridization

  • Chromogenic in situ hybridization
  • Hybridization performed on a DakoCytomation Slide Hybridizer

Tissue Microarray

  • Tissue microarrays can be created with any combination of human tissue, animal tissues or cell culture pellets
  • Arrays are constructed using the Beecher MTA-I
  • Tissue core sizes can range from 0.6 mm to 2.0 mm, providing from 40 to over 200 sample cores in one paraffin block
  • Microarray slides can be stained using a variety of histochemical or immunohistochemical reactions

 

DNA Extraction

  • DNA extraction from formalin-fixed paraffin embedded (FFPE) tissue sections suitable for PCR
Laser Capture Microdissection
 

The MMPRCL now has laser capture microdissection available located in C7718 using the Zeiss PALM Microbeam. With this instrument an experienced investigator is capable of isolating individual cell, tissues, or stromal matrix from either frozen and paraffin sections. It is an ideal method to insure the purity of the starting material for downstream proteomic and genomic applications in the common situation where analyses of whole tissue homogenates will yield imprecise results due to the heterogeneity of tissue or cell types. The lab is capable of operating on an RNAse free level to insure high quality samples for genomic studies.

Gene Expression Array

(under negotiation, probable availability, November, 2008)
 

Tissue Bank

(located in the Molecular & Histopathology Core, C7718B)
See:  http://www.pennstatehershey.org/web/cancer/research/facilities/tissue-bank for more detail regarding available specimens, application forms, etc.
 
As of August 2009:
Tissues available ~9500
     tumor ~2500
     plasma, buffy coat, RBC ~23,000
     buccal smears ~1650
Tissues distributed ~ 2200 to 42 researchers