Facilities

Division of Artificial Organs
Laboratory. Maintains a polymer fabrication lab (700 square feet), system assembly clean room (260 square feet), machine shop (1,000 square feet), electronics lab (200 square feet), mock loop test lab (630 square feet), mechanical assembly room (120 square feet), & computer aided design room (120 square feet) in the Biomedical Research Building. Dr. Siedlecki's laboratory has 1200 square feet of space for labeling and analysis of blood sacs and retrieved samples. The Hershey Medical Center maintains a 1000 square feet clinical Hematology, Coagulation, and Thrombosis Laboratory section that also supports our research mission. Statistical analysis support is available through the Department of Health Evaluation Sciences.

Animal. The large animal wing of the Animal Research Facility has surgery and surgical support facilities (2000 sq.ft.), two animal care rooms (1250 square feet), a radiology suite, holding rooms, and a necropsy room. Office and storage space (800 square feet) are also available. The Comparative Medicine Department includes histopathology, clinical chemistry, and microbiology labs to support research. The diagnostic labs provide rapid hematology, clinical chemistry, serologic and histologic support, and diagnostic bacteriological studies, including antibiotic sensitivity testing for each species of lab animal. A staff of veterinarians trained in lab animal medicine and veterinary pathology provides professional support.

Computer. Computer-aided design tools and data acquisition systems in the Division of Artificial Organs are PC-based. Licensed software includes Solidworks, Autodesk, ANSYS RMXprt and Maxwell, LabView, Matlab, and Mathcad. Several stand-alone PC's are available for controller software development, data acquisition and analysis, and monitoring of in vivo and in vitro experiments.

Office. Adequate office space is available for conferences, analysis of research data, and manuscript preparation. PicTel facilities are available for conferencing between the University Park and Hershey campus.

Division of Colorectal Surgery
Laboratory. Is located on the 4th floor of Biomedical Research Building. Our laboratory is state-of-the-art and is well-equipped for all aspects of biochemical, molecular biology, and molecular genetics research. The laboratory space has a total of 1983 square feet, in 4 rooms.

Room C4814 includes approximately 895 square feet of laboratory space and access to a shared (with 4 additional investigators) common equipment space of 185 square feet All of the standard laboratory equipment (i.e., vortexes, stirrers, pH meter, balances, etc.), an array of molecular biology equipment including electrophoresis apparatus and power supplies, equipment for western, blotting, agarose and PAGE gel electrophoresis (Multiphor System, Amersham, Denville Scientific), Additional large equipment including a laminar flow tissue culture hood and adjacent CO2 incubator, chemical fume hood, thermal cycler (Thermo electron Px2), standard and inverted microscopes (Nikon), microcentrifuges, a temperature-adjustable high-speed tabletop centrifuges, incubators, rockers, UV transilluminator and documentation camera, 4oC refrigerator, –20oC freezer, –80oC freezer, two liquid nitrogen tank for cell storage, and two Pentium 4 computer systems with attached printers and full access to high-speed internet and Penn State networking.

Room C4819 is 384 square feet and an attached 80 square feet office room with 2 Pentium computer systems with attached inkjet printer. One special area is for surgery of small animals such as mice and rat.
Rooms C4816 & C4818 have a combined 1,160 square feet, with four office rooms.

Division of Otolaryngology
Zubar Temporal Bone Laboratory: We have a dedicated temporal bone laboratory at the College of Medicine, consisting of a space with a total of 413.9 square feet. This space is a fully equipped temporal bone lab. Our laboratory in room C4821 contains an anteroom where there are 8 temporal bone stations. Each is equipped with connections for air, water, and vacuum as well as an operating microscope. In addition, in the back room (C4821A), there is a 9th temporal bone station, with the same hook ups, as well as wiring to allow for digital display of the microscope view to the anteroom. This allows for the ability to display a drilled specimen. Additionally, the back room has some laboratory facilities, including a hood and a bench. There is also a full size freezer as well as a refrigerator for housing specimens.

Division of General Surgery
Laboratories – We have two dedicated laboratories, one at the College of Medicine (COM; Department of Surgery) and one at the University Park (UP; Department of Nutritional Sciences). The laboratories are electronically connected using Smartboard technology and plasma panels. Our dedicated laboratories at COM consist of 2 separate spaces with a total of 2142 square feet which is shared with another investigator. This space is a fully equipped biochemistry, cell and molecular biology and surgical facility which contains adequate bench and desk space to facilitate the work of ~15 individuals. Our laboratory contains an anteroom which is dedicated office space. We have a large laminar flow hood for cell, virus and bacterial culture and procedures utilizing human tissues, 3, 37ºC incubators, nitrogen-vapor cell storage, 3 large fume hoods for toxic and radioactive compounds. We are authorized by the Pennsylvania State University to use radioisotopes and biohazardous (BSL2) organisms. In addition to routine laboratory equipment, we have equipment to perform molecular methodologies (thermocycler, shakers, incubators, radiolabeling, detection, gradient PCR, etc.), a microplate reader for visible/UV/nanodrop, microplate fluorometer (end-point and kinetics), a chemiluminescence detection/densitometry system, dark room housing an advanced microscope and imaging system capable of ratiometiric imaging in real time (up to 100X under oil; inverted, bright-field, fluorescence, live cell, 500 frames per second), autoclave, centrifuges (microultra-, high-speed, micro-). Departmental resources that are shared include glass washing/autoclaving, real-time PCR thermocycler, multiple centrifuges (ultra-, high-speed), gamma and beta scintillation counters for the sensitive detection of radioisotopes, a darkroom and developing equipment, walk-in refrigerator, numerous biofreezers for sample storage.

Our dedicated laboratory at UP consists of a large space with approximately 2000 square feet with 3 separate anterooms and a walk-in refrigerator. This space is a fully equipped biochemistry, cell and molecular biology facility which contains adequate bench and desk space to facilitate the work of ~15 individuals. Our laboratory contains an anteroom dedicated to cell culture with 2 large laminar flow hoods for cell, virus and bacterial culture and procedures utilizing human samples, 37ºC incubators, nitrogen-vapor cell storage; an anteroom dedicated to radioisotope use; an anteroom dedicated to chemical and and sample storage. Our lab contains 2 large fume hoods for toxic and radioactive compounds. In addition to routine laboratory equipment, we have equipment to perform molecular methodologies (thermocycler, shakers, incubators, radiolabeling, detection, etc.), multiple centrifuges (microultra-, high-speed, micro-), a microplate reader for visible/UV/nanodrop, microplate fluorometer (end-point and kinetics), luminometer, a chemiluminescence detection/densitometry system, microscope and imaging system (up to 100X under oil; inverted, bright-field, fluorescence). We are authorized by the Pennsylvania State University to use radioisotopes and biohazardous (BSL2) organisms. Departmental resources that are shared include real-time PCR thermocycler, phoshoimager, multiple HPLC systems for the separation and measurement of various molecules, multiple centrifuges (ultra-, high-speed), gamma and beta scintillation counters for the sensitive detection of radioisotopes, a darkroom and developing equipment. We are fully equipped to perform. trace mineral analysis by atomic absorption spectrophotometry and ICP-MS.

Office – Dr. Soybel has an office suite with approximately 320 square feet in addition to the anteroom described as office space in the laboratory. The office is located in the fourth floor of the main building of the Penn State Milton S. Hershey Medical Center, room C4630. At UP a separate office is located in the Chandlee Laboratory on the second floor, room 223. The office is approximately 120 square feet and he is located at this office one day a week.

Division of Plastic Surgery
Graham Laboratory: The laboratory consists of a well-equipped 1000 square foot laboratory. The main laboratory has 4 rooms: adjoining 90 square feet technician's office, 90 square feet microscope room, 120 square feet. Cell Culture room, and 80 square feet rodent animal surgery room. The laboratory has 4 bench top areas: 1) Western blot, 2) RNA, 3) Northern blot, and 4) Immunohistochemistry and work space for occupants.

Office/ Meeting Space
The PI has a separate office located within the Division of Plastic Surgery. The Divisions of Plastic Surgery has a library/conference room with generous space for group meetings between key personnel.

Division of Surgical Oncology
Laboratory and Facilities has approximately 1000 square feet of oncology laboratory bench space (C5525, C5529) opposite the faculty offices, and adjacent to the Penn State Hershey Cancer Institute. Within the laboratory there is a walk-in cold-room (C5529C 50 square feet), cell culture room (C5529B 110 square feet.) and facility, fume hood radioisotope handling facilities. In addition, there is a shared darkroom, and all other common equipment required in a modern nucleic acid and protein chemical laboratory. Routinely used equipment is available in his Laboratory, such as Forma* Series II 3110 Water-Jacketed CO2 Incubators (3), Ultra Low Freezers To -85° C, Reach-In Refrigerator with Glass Door, -20°C Freezer, CRYO Biological Storage System,  Nikon Eclipse TS100 Inverted microscope, Nikon Eclipse E 100 Inverted microscope, Leica M3Z 1000X Magnification Stereomicroscope, Laminar Flow Workstation from Clean Air Products, Robbins Scientific 2000 Micro Hybridization Incubator, Techne TC-412 Thermal Cycler, Sorvall RT7 Benchtop Centrifuge, Allegra® 6R Benchtop Centrifuge, Jouan A-14 Centrifuge, VWR 300V Power Supply, EC 105 power supply, Stratagene UV Stratalinker 1800, Heraeus* Function Line Microbiological Incubators, Benchtop UV Transilluminators, Standard Infusion Only Harvard Pump 11 Plus Syringe Pumps, Sartorius CP Analytical Balances (model CP64), Sharp® Mid-size Carousel Microwave, VWR® Incubating/Cooling Shaker from VWR etc.

Institutional Core Research Facilities
Core Facilities – Our shared Technological Facilities (http://www.pennstatehershey.org/web/college/research/core) include but are not limited to Flow Cytometry Core, Molecular Genetics Core, Function Genomics Core, Macromolecular Core Facility, and Proteomics Core. COM houses a biomedical library with education resources that include electronic access to a wide array of peer reviewed journals. We have access to the shared facilities of the Huck Institute (http://www.huck.psu.edu/stf/home.html) and the Metabolomics Core (http://www.cmtc.psu.edu/patterson/facilities.asp) at University Park, State College, PA.

Flow Cytometry Core Facility (FCCF) – The clinically certified facility contains four flow cytometers dedicated to research, including a 4-color FACSCalibur, an 8-color FACSCanto, a 15-color LSR II, and a 16-color FACSAria SORP high speed cell sorter, all available for use by investigators at the College of Medicine.

Penn State Molecular Genetics Core Facility (MGCF) – provides automated DNA sequencing and genotyping, using an ABI 3100 capillary sequencer, and ABI 377 DNA Sequencer, an MJ Research thermocycler, and a PE Applied Biosystems GeneAmp PCR System 9700 thermocycler.

Functional Genomics Core Facility (FGCF) – has capacity for high throughput genotyping. Quantitative Real-time PCR systems include the Applied Biosystems 7900HT and 7300 qRT-PCR analyzers which are available for use by individual investigators using associated Primer Express and Sequence Detection Software. Agilent BioAnalyzer 2100 is used to assess the quality and quantity of DNA, RNA, and protein samples. Biosystems Prism 7700 available for QRT-PCR with Primer Express and Sequence Detection software.

Bioinformatics Consulting Center (BCC) – maintains computer and software resources for routine sequence analysis, provides services for raw sequence data processing, sequence assembly and preliminary annotation as well as assistance in sequence analysis, including BLAST search, protein domain analysis, multiple sequence alignment, phylogenetic analysis, and other common sequence-based methods. Where high-throughput analysis is required, the BCC offers development or local implementations of automated data analysis pipelines and data management systems. This support ranges from short consultation to long-term research collaboration leading to joint grant proposals.

Macromolecular Core Facility – includes protein/peptide sequencing using the Applied Biosystems Procise 491 and Applied Biosystems 477A protein sequencer; peptide synthesis using the One Milligen 9050 and the Perseptive Biosystems 9050 Fmoc peptide synthesizer; oligonucleotide synthesis using the One Perseptive Biosystems Expedite 8905 and Expedite 8909 DNA synthesizer, and purification using Nensorb columns, MALDI-TOF Mass Spectroscopy using an Applied Biosystems 4700 Proteomics Analyzer MALDI TOF-TOF and a Perseptive Biosystems DE-PRO reflectron MALDI-TOF; Image analysis using a Molecular Dynamics Phosphorimager and a Molecular Dynamics Densitometer are coupled to a Sun SPARC II workstation for sophisticated analysis of radiometric and densitometric images.

Proteomics Facility – includes an Applied Biosystems 4700 Proteomics Analyzer MALDI TOF-TOF, Perseptive Biosystems DE-PRO reflectron MALDI-TOF, Advanced GPS Explorer software from Applied Biosystems, Typhoon 9410 imager, DeCyder 6.0 software (GE Healthcare) and robotic gel spot picker (Ettan Picker, GE Healthcare).

Other
The University houses a biomedical library, a machine and electronic shop, educational resources such as the G.T. Harrell Library, and professional services for graphics and photography. These additional resources are available Monday through Friday 8AM – 5 PM. The G. T. Harrell Library is available Monday through Thursday 8AM – midnight, Friday and Saturday, 8AM – 10PM. The Department of Surgery provides secretarial services and administers purchasing and budgetary matters and is available Monday through Friday 7:30 AM – 5PM. The Division of Outcomes, Research and Quality, Department of Surgery, provides support to all faculty for grant preparation and submission, IRB submission, and manuscript formatting and submission and is available Monday through Friday 8AM – 4:30 PM.  Additionally, they offer statistical and database services as needed.